For more information on things to do in Crystal River or Kings Bay visit Discover Crystal River.   


  • The refuge at Three Sisters Springs is open 7 days a week, 8:30 am - 4:30 pm, last entrance at 3:30 pm.
  • Tickets can be purchased at the Three Sisters Springs Center or the front gates to the refuge at Three Sisters Springs.
  • Admission ends 1 hour before closing.
  • Trolley tours are available Friday - Sunday from April 1 - November 14, and 7 days a week November 15 - March 31.
  • Tours depart every 30 mins from the Three Sisters Springs Center 8:30 - 3:30pm (located 1 mile from the springs, behind Crystal River City Hall). Last pick up is at 4:30pm. 

Click here to view Calendar for scheduled holidays and other closures.

Boardwalk at Three sisters Springs.

Land Access at Three Sisters Springs


Three Sisters Springs, a unit of the Crystal River National Wildlife Refuge (CRNWR), is the gem of the Nature Coast. Visitors to the refuge can enjoy wildlife viewing, hiking trails, bicycling and guided  tours lead by US Fish & Wildlife Service (USFWS) volunteers. The boardwalk has viewing platforms to observe the springs and native wildlife. There are also nature trails that offer views of Magnolia Springs, Lake Crystal and the wetlands. Three Sisters Springs is a world-renowned winter sanctuary for the West Indian Manatee. Over 100 different native and migratory bird species use the refuge year-round.


In an effort to preserve the shoreline vegetation, control erosion and maintain water clarity, CRNWR does not allow water access to visitors from  this site, nor do they allow land access from the water.

  •  Land access is open daily from 8:30 am - 4:30 pm, no new visitors are admitted after 3:30 pm 
  • There is no parking permitted on the refuge, however, there are a limited number of handicapped parking space available with valid permit.
  • Pets are not permitted on the refuge, registered service animals are welcome.
  • Restroom facilities consists of port-a -potties, no potable water on site.
  • Leave only footprints, take only pictures. Leave rocks, plants and other natural features as you find them so they can be enjoyed by future generations.


Trolley to Three Sisters Springs


Trolley availability is based on the season:

  • April 1 - November 14: Trolley operates on Friday, Saturday and Sunday only
  • November 15 - March 31: Trolley operates 7 days a week

Trolley departs from the Three Sisters Springs Center every 30 minutes to the boardwalk at Three Sisters Springs. Additional  stops are made at Hunter Springs Park, South Citrus Ave., and Heritage Village.


Guided Walking Tours


Crystal River National Wildlife Refuge Volunteers lead guided tours at Three Sisters Springs daily during manatee season (November 14th-March 31st). Please contact the CRNWR  for information on guided walking tours, 352-563-2088.






Three Sisters Springs Center

Official Concessionaire for Land Access to the Crystal River National Wildlife Refuge's Three Sisters Springs

123 NW US Highway 19

City of Crystal River, 34428

Click here for Google Map

Ph: (352) 586-1170 
Contact Us

8:30am - 4:30pm EST

Ticket sales end 1 hour before closing

See Calendar for Closures

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